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5O3E

Human Brd2(BD2) mutant in complex with Me-Am1

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyCCD
Collection date2014-11-12
DetectorRIGAKU SATURN 944+
Wavelength(s)1.5418
Spacegroup nameP 21 21 2
Unit cell lengths52.177, 71.950, 31.917
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution35.975 - 1.400
R-factor0.1331
Rwork0.132
R-free0.16530
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4qeu
RMSD bond length0.006
RMSD bond angle1.675
Data reduction softwareiMOSFLM
Data scaling softwareAimless
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.9801.450
High resolution limit [Å]1.4001.400
Rmerge0.1010.223
Number of reflections243372352
<I/σ(I)>15.07
Completeness [%]99.897.92
Redundancy9.64.8
CC(1/2)0.9980.955
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1M Tris pH 7.75 55% Pentaerythritol propoxylate (5/4 PO/OH) 0.2M Imidazole pH 8

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