5O3E
Human Brd2(BD2) mutant in complex with Me-Am1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-12 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.177, 71.950, 31.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.975 - 1.400 |
R-factor | 0.1331 |
Rwork | 0.132 |
R-free | 0.16530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qeu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.675 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.980 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.101 | 0.223 |
Number of reflections | 24337 | 2352 |
<I/σ(I)> | 15.07 | |
Completeness [%] | 99.8 | 97.92 |
Redundancy | 9.6 | 4.8 |
CC(1/2) | 0.998 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris pH 7.75 55% Pentaerythritol propoxylate (5/4 PO/OH) 0.2M Imidazole pH 8 |