5O3C
Human Brd2(BD2) mutant in complex with 9-Me
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 31.852, 52.082, 71.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.852 - 1.600 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qeu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.850 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.111 | 0.563 |
| Number of reflections | 16285 | 1575 |
| <I/σ(I)> | 7.67 | 2.04 |
| Completeness [%] | 99.5 | 99.56 |
| Redundancy | 4.3 | 4.2 |
| CC(1/2) | 0.992 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Tris pH 8.5 60% Pentaerythritol propoxylate (5/4 PO/OH) |
