5O39

Human Brd2(BD2) mutant in complex with ME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-31
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	52.406, 72.129, 31.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.769 - 1.740
R-factor	0.1464
Rwork	0.144
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qeu
RMSD bond length	0.007
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.770	1.802
High resolution limit [Å]	1.740	1.740
Rmerge	0.049	0.056
Number of reflections	12633	291
<I/σ(I)>	43.16
Completeness [%]	97.1	79.28
Redundancy	17.1	15.3
CC(1/2)	1.000	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	293	0.2M Tris pH9 53% Pentaerythritol propoxylate (5/4 PO/OH)