5O33

A structure of the GEF Kalirin DH1 domain in complex with the small GTPase Rac1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92819
Spacegroup name	P 65 2 2
Unit cell lengths	63.255, 63.255, 346.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.780 - 1.640
R-factor	0.2084
Rwork	0.207
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nz8
RMSD bond length	0.009
RMSD bond angle	1.072
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.780
High resolution limit [Å]	1.640
Rmerge	0.097
Number of reflections	52137
<I/σ(I)>	11.21
Completeness [%]	100.0
Redundancy	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1M citrate pH4.2, 0.2M sodium chloride, 18% (W/V) PEG 8000