5O2A
FolD Q98H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.106, 79.393, 101.776 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 46.562 - 1.900 |
| R-factor | 0.1666 |
| Rwork | 0.166 |
| R-free | 0.18120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dia |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.102 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.540 |
| Number of reflections | 113661 | |
| <I/σ(I)> | 22.1 | 4.4 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 9.7 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 Ammonium Sulfate, Bis-Tris pH 6.0 and 25 % PEG 3350 |
