5O1O
Crystal structure of human aminoadipate semialdehyde synthase, saccharopine dehydrogenase domain with proline bound.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.374, 102.414, 142.074 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.080 - 2.480 |
| R-factor | 0.32304 |
| Rwork | 0.322 |
| R-free | 0.34754 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l76 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.923 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.030 | 2.760 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.113 | |
| Number of reflections | 37204 | 1792 |
| <I/σ(I)> | 12.6 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.6 | 6.9 |
| CC(1/2) | 0.998 | 0.615 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 286 | 0.1M tris pH 7.5 -- 0.2M sodium malonate -- 20% PEG3350 |
