5NZH
Crystal structure of UDP-glucose pyrophosphorylase V402W mutant from Leishmania major
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.560, 70.890, 112.290 |
| Unit cell angles | 90.00, 123.54, 90.00 |
Refinement procedure
| Resolution | 47.854 - 2.900 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oef |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.854 | |
| High resolution limit [Å] | 2.900 | |
| Number of reflections | 26339 | |
| <I/σ(I)> | 4.37 | 2.14 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 2.25M ammonium sulfate, 100mM Tris-HCl pH 7.2, 0.1% Tween 80 |
