5NVM
Crystal structure of the human 4EHP-GIGYF2 complex lacking the auxiliary sequences
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.210, 98.590, 39.280 |
| Unit cell angles | 90.00, 99.59, 90.00 |
Refinement procedure
| Resolution | 44.613 - 2.000 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nvl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.770 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.613 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.611 | |
| Number of reflections | 38577 | |
| <I/σ(I)> | 12 | 2.09 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium acetate pH 4.6 0.6 M diammonium phosphate |
