5NVK
Crystal structure of the human 4EHP-GIGYF1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999810 |
Spacegroup name | P 42 |
Unit cell lengths | 135.320, 135.320, 60.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.843 - 2.900 |
R-factor | 0.2067 |
Rwork | 0.204 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nvl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.494 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.843 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 24699 | |
<I/σ(I)> | 10.9 | 1.95 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG 3350 0.2 M KCl |