5NSQ
Structure of Leucyl aminopeptidase from Trypanosoma brucei in complex with Actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.909, 165.730, 121.980 |
| Unit cell angles | 90.00, 112.45, 90.00 |
Refinement procedure
| Resolution | 41.500 - 3.000 |
| R-factor | 0.26617 |
| Rwork | 0.265 |
| R-free | 0.29641 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nsk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.443 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.116 | 0.440 |
| Number of reflections | 68219 | |
| <I/σ(I)> | 4.5 | 2 |
| Completeness [%] | 97.0 | 98 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 8.0, 2.4 M (NH4)2SO4, 5 mM MnSO4, 5 mM actinonin |
