5NSM
unliganded Structure of Leucyl aminopeptidase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.368, 143.760, 270.085 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 32.730 - 2.400 |
| R-factor | 0.20689 |
| Rwork | 0.206 |
| R-free | 0.22508 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nsk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.730 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.117 | 0.351 |
| Number of reflections | 244412 | |
| <I/σ(I)> | 5.7 | 2 |
| Completeness [%] | 94.5 | 88.7 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 6.8, 0.2 M MgCl2, 11 % (w/v) PEG 8000, 10 microM ZnCl2, 10 mM L-leucine |
