5NSM
unliganded Structure of Leucyl aminopeptidase from Trypanosoma brucei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.368, 143.760, 270.085 |
Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
Resolution | 32.730 - 2.400 |
R-factor | 0.20689 |
Rwork | 0.206 |
R-free | 0.22508 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nsk |
RMSD bond length | 0.014 |
RMSD bond angle | 1.397 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.730 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.117 | 0.351 |
Number of reflections | 244412 | |
<I/σ(I)> | 5.7 | 2 |
Completeness [%] | 94.5 | 88.7 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 6.8, 0.2 M MgCl2, 11 % (w/v) PEG 8000, 10 microM ZnCl2, 10 mM L-leucine |