5NQ0
Porcine (Sus scrofa) Major Histocompatibility Complex, class I, presenting DFEREGYSL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.616, 46.340, 66.735 |
| Unit cell angles | 104.36, 101.21, 102.20 |
Refinement procedure
| Resolution | 39.270 - 1.100 |
| R-factor | 0.18999 |
| Rwork | 0.189 |
| R-free | 0.20852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qq3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.127 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.280 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.086 | 0.179 |
| Number of reflections | 170825 | 11419 |
| <I/σ(I)> | 6 | 1.1 |
| Completeness [%] | 92.3 | 83.1 |
| Redundancy | 1.9 | 1.7 |
| CC(1/2) | 0.998 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PACT screen, condition A09: 0.2 M LiCl, 0.1 M Sodium Acetate, pH 5.0, 20% PEG 6000 |
