5NM5
Tubulin Darpin room-temperature structure in complex with Colchicine determined by serial millisecond crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-06-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.920, 85.020, 84.340 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 31.000 - 2.050 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4drx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 62424 | 3355 |
| <I/σ(I)> | 5.19 | 0.52 |
| Completeness [%] | 91.9 | 49.45 |
| Redundancy | 194.5 | 6.2 |
| CC(1/2) | 0.990 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-24% PEG 3350, 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris Methane pH5.5 |
