5NG0

Structure of RIP2K(L294F) with bound AMPPCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	94.180, 94.180, 201.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.17134
Rwork	0.170
R-free	0.19143
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fb8
RMSD bond length	0.040
RMSD bond angle	1.338
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.250
High resolution limit [Å]	2.000
Rmerge	0.035
Number of reflections	54007
<I/σ(I)>	19.9
Completeness [%]	92.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Hepes 100 mM pH 7.5, 1 mM MgCl2, 2.0 M LiCl and 5% PEG 6000