5NFU
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LHSpTA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.197, 59.808, 58.256 |
Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
Resolution | 41.199 - 1.809 |
R-factor | 0.1699 |
Rwork | 0.168 |
R-free | 0.20200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.282 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.808 | 1.815 |
High resolution limit [Å] | 1.809 | 1.809 |
Rmerge | 0.448 | |
Rpim | 0.044 | 0.314 |
Number of reflections | 21491 | 531 |
<I/σ(I)> | 3.7 | 2.2 |
Completeness [%] | 99.3 | 90.8 |
Redundancy | 3.7 | 2.7 |
CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M HEPES 7 30% Jeffamine ED-2001 |