5NFO

Human JMJD7 in complex with Mn and 2OG in the P21212 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	137.315, 61.020, 86.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.776 - 2.173
R-factor	0.1728
Rwork	0.171
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nfn
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.776	2.230
High resolution limit [Å]	2.170	2.170
Rmerge	0.131	1.176
Number of reflections	38951
<I/σ(I)>	13.2857	1.75
Completeness [%]	100.0	100
Redundancy	9	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	26 mg/mL His6-JMJD7-R260C, 2 mM MnCl2, 4 mM 2OG, 1.5 M Sodium Nitrate, 0.1 M Sodium Acetate pH 4.6 and additives (0.25% beta-NADP, 0.25% ATP, 0.25% N-Acetyl-D-galactosamine, 0.25% Gentamicin sulfate, 0.02 M HEPES-Na pH 6.8)