5NDH

The structure of the G. violaceus guanidine II riboswitch P2 stem-loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	48.727, 57.825, 100.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.166 - 1.810
R-factor	0.2075
Rwork	0.207
R-free	0.22220
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	2.055
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2219: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.170	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.059	1.410
Rpim	0.026	0.660
Number of reflections	49436
<I/σ(I)>	16.2	1.1
Completeness [%]	100.0	98.6
Redundancy	6.3	6.4
CC(1/2)	0.998	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate