5NDH
The structure of the G. violaceus guanidine II riboswitch P2 stem-loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92011 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.727, 57.825, 100.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.166 - 1.810 |
R-factor | 0.2075 |
Rwork | 0.207 |
R-free | 0.22220 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 2.055 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.170 | 1.840 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.059 | 1.410 |
Rpim | 0.026 | 0.660 |
Number of reflections | 49436 | |
<I/σ(I)> | 16.2 | 1.1 |
Completeness [%] | 100.0 | 98.6 |
Redundancy | 6.3 | 6.4 |
CC(1/2) | 0.998 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate |