5NCO
Quaternary complex between SRP, SR, and SecYEG bound to the translating ribosome
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.8 |
| Number of Particle | 1029950 |
| Software | EMAN,RELION,PHENIX,EPU,CTFFIND,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
