5NAN

Crystal Structure of human IL-17AF in complex with human IL-17RA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.999920
Spacegroup name	P 1 2 1
Unit cell lengths	100.002, 66.103, 104.119
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	66.100 - 3.300
R-factor	0.189
Rwork	0.186
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4HSA
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.100	66.100	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.149	0.029	3.483
Rmeas	0.178	0.034	4.151
Total number of observations	69444
Number of reflections	20621	241	1494
<I/σ(I)>	5.71	27.23	0.32
Completeness [%]	99.1	92	99.5
Redundancy	3.368	3.17	3.337
CC(1/2)	0.997	0.999	0.184

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.1M HEPES, 15% PEG MME 5,000, 0.05M Ammonium Acetate