5N9J
Core Mediator of transcriptional regulation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97940, 1.90750, 0.97794, 0.97863 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 145.864, 211.768, 267.028 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 98.486 - 3.400 |
R-factor | 0.2343 |
Rwork | 0.233 |
R-free | 0.25600 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 98.486 | 98.486 | 3.600 |
High resolution limit [Å] | 3.400 | 10.150 | 3.400 |
Rmerge | 0.058 | 0.027 | 1.644 |
Rmeas | 0.063 | 0.030 | 1.784 |
Number of reflections | 113813 | 4578 | 17800 |
<I/σ(I)> | 17.05 | 54.15 | 1.06 |
Completeness [%] | 99.5 | 99.3 | 97.5 |
Redundancy | 6.643 | 5.704 | 6.622 |
CC(1/2) | 0.999 | 0.998 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 293 | 0.1 M MES pH 6.5, 3.2% PEG 20000, 700 mM ammonium nitrate |
1 | EVAPORATION | 6.5 | 293 | 0.1 M MES pH 6.5, 3.2% PEG 20000, 700 mM ammonium nitrate |