5N92
Crystal Structure of Human IL-17AF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.999970 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 87.380, 87.380, 126.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.2071 |
Rwork | 0.205 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 15.000 | 15.000 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.130 | 0.029 | 1.493 |
Rmeas | 0.136 | 0.030 | 1.554 |
Total number of observations | 278250 | ||
Number of reflections | 22302 | 311 | 1611 |
<I/σ(I)> | 14.67 | 41.86 | 1.75 |
Completeness [%] | 99.5 | 100 | 100 |
Redundancy | 12.476 | 10.232 | 13.171 |
CC(1/2) | 0.999 | 1.000 | 0.721 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1M Bis-Tris-Propane, 0.5M Succinic acid, pH 7.0 |