5N6W
Retinoschisin R141H Mutant
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.2 |
| Software | EMAN,RELION,Flex-EM,CTFFIND,DockEM |
| Symmetry | D8 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
