5N09

Crystal structure of L107C/A313C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 2 21 21
Unit cell lengths	58.902, 180.598, 205.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.991 - 3.900
R-factor	0.3064
Rwork	0.304
R-free	0.34680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4utb
RMSD bond length	0.005
RMSD bond angle	1.281
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.270
High resolution limit [Å]	3.900	3.900
Rmerge	0.686	1.965
Rpim	0.354	1.044
Number of reflections	19970	23344
<I/σ(I)>	2.4	0.7
Completeness [%]	96.9	98
Redundancy	5.1	4.9
CC(1/2)	0.891	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100mM Tris pH 8.5 20.7% PEG 4K