5N09
Crystal structure of L107C/A313C covalently linked dengue 2 virus envelope glycoprotein dimer in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 A11
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Envelope Glycoprotein E | polymer | 430 | 47467.2 | 2 | UniProt (C3VXD1) UniProt (by SIFTS) (A0A0B4SHY9) | Dengue virus 2 | |
| 2 | C, D (H, I) | BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11 - Heavy chain | polymer | 283 | 30355.5 | 2 | Homo sapiens (Human) | ||
| 3 | E, F (L, M) | BROADLY NEUTRALIZING HUMAN ANTIBODY EDE2 A11 - Light chain | polymer | 218 | 23107.7 | 2 | Homo sapiens (Human) | ||
| 4 | G, H (C, D) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1057.0 | 2 | In PDB GlyTouCan (G82348BZ) | |||
| 5 | I (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 6 | J, K (A, H) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 395 (UniProt: C3VXD1)
| PDB | External Database | Details |
|---|---|---|
| Cys 107 | Leu 387 | engineered mutation |
| Lys 118 | Met 398 | conflict |
| Cys 313 | Ala 593 | engineered mutation |
| Leu 396 | - | expression tag |
| Val 397 | - | expression tag |
| Pro 398 | - | expression tag |
| Arg 399 | - | expression tag |
| Gly 400 | - | expression tag |
| Ser 401 | - | expression tag |
| Ser 402 | - | expression tag |
| Ala 403 | - | expression tag |
| Trp 404 | - | expression tag |
| Ser 405 | - | expression tag |
| His 406 | - | expression tag |
| Pro 407 | - | expression tag |
| Gln 408 | - | expression tag |
| Phe 409 | - | expression tag |
| Glu 410 | - | expression tag |
| Lys 411 | - | expression tag |
| Gly 412 | - | expression tag |
| Gly 413 | - | expression tag |
| Ser 414 | - | expression tag |
| Gly 415 | - | expression tag |
| Gly 416 | - | expression tag |
| Gly 417 | - | expression tag |
| Ser 418 | - | expression tag |
| Gly 419 | - | expression tag |
| Gly 420 | - | expression tag |
| Ser 421 | - | expression tag |
| Ala 422 | - | expression tag |
| Trp 423 | - | expression tag |
| Ser 424 | - | expression tag |
| His 425 | - | expression tag |
| Pro 426 | - | expression tag |
| Gln 427 | - | expression tag |
| Phe 428 | - | expression tag |
| Glu 429 | - | expression tag |
| Lys 430 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 201860.7 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 2113.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 221.2 | |
| All* | Total formula weight | 204195.8 |
