5MZA
The DBLb domain of PF11_0521 PfEMP1 bound to human ICAM-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.639, 109.830, 112.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.190 - 2.780 |
R-factor | 0.2063 |
Rwork | 0.205 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.970 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 78.700 |
High resolution limit [Å] | 2.780 |
Number of reflections | 22210 |
<I/σ(I)> | 8.2 |
Completeness [%] | 99.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% (w/v) PEG 20000, 20% (v/v) PEG 500 and 0.1M Tris-BICINE (pH 8.5) |