5MVN
X-ray structure of the M205W mutant of GLIC in complex with propofol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-05-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.229, 132.474, 159.759 |
Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
Resolution | 49.280 - 3.490 |
R-factor | 0.2183 |
Rwork | 0.218 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hfi |
RMSD bond length | 0.009 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.490 |
Number of reflections | 33576 |
<I/σ(I)> | 3.5 |
Completeness [%] | 98.0 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 400 mM NaSCN 100 mM NaAcetate pH4 12-15 % PEG4K 16 % glycerol 2 % DMSO |