5MVM
X-ray structure of the F14'A -N15'A double mutant of GLIC in complex with propofol
Replaces: 4HFCExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-04-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.760, 132.510, 160.520 |
Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.1958 |
Rwork | 0.195 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hfb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 66139 |
<I/σ(I)> | 7.6 |
Completeness [%] | 98.0 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 400mM NaSCN 100mM NaAcetate pH4 12-15% PEG4K 16% glycerol 2% DMSO |