5MVM
X-ray structure of the F14'A -N15'A double mutant of GLIC in complex with propofol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-04-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.760, 132.510, 160.520 |
| Unit cell angles | 90.00, 102.72, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.1958 |
| Rwork | 0.195 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 66139 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 98.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 400mM NaSCN 100mM NaAcetate pH4 12-15% PEG4K 16% glycerol 2% DMSO |
