5MO9

Structure of human TrkB receptor ligand binding domain in complex with the Fab frgment of antibody AB20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0005
Spacegroup name	P 21 21 2
Unit cell lengths	148.370, 55.430, 69.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.000 - 2.594
R-factor	0.182
Rwork	0.190
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcf
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	XDS (May 1, 2016 BUILT=20160617)
Data scaling software	Aimless (0.5.27)
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.402	2.639
High resolution limit [Å]	2.594	2.594
Rmerge	0.158	0.963
Number of reflections	18392
<I/σ(I)>	11	2.2
Completeness [%]	100.0	99.9
Redundancy	6.4	6.6
CC(1/2)	0.995	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	6% w/v PEG 3350, 25 mM Tris pH 8.5