5MLE
Crystal Structure of Human Dihydropyrimidinease-like 2 (DPYSL2A)/Collapsin Response Mediator Protein (CRMP2 13-516) Mutant Y479E/Y499E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.900, 185.820, 196.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.980 - 2.480 |
| R-factor | 0.20851 |
| Rwork | 0.206 |
| R-free | 0.25408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gse |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.667 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 83.980 |
| High resolution limit [Å] | 2.480 |
| Number of reflections | 46370 |
| <I/σ(I)> | 6.21 |
| Completeness [%] | 99.9 |
| Redundancy | 6.8 |
| CC(1/2) | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | (0.2M magnesium chloride, 25% PEG 3350 and 0.1M bis-tris buffer pH 6.5 |
