5MLB
Crystal structure of human RAS in complex with darpin K27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.927 |
| Spacegroup name | P 65 |
| Unit cell lengths | 197.140, 197.140, 84.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.570 - 3.220 |
| R-factor | 0.20264 |
| Rwork | 0.201 |
| R-free | 0.23537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.982 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.570 | 3.300 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.362 | 0.965 |
| Number of reflections | 30646 | |
| <I/σ(I)> | 7.8 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 9.9 |
| CC(1/2) | 0.988 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% PEGMME 2000, 299mM Calcium Acetate, 100mM Sodium Cacodylate pH 6.5 |
