5MI0
A thermally stabilised version of Plasmodium falciparum RH5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.656, 85.547, 132.873 |
Unit cell angles | 90.00, 90.91, 90.00 |
Refinement procedure
Resolution | 32.910 - 2.350 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.770 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.096 | 0.554 |
Number of reflections | 36539 | |
<I/σ(I)> | 13.9 | 3.1 |
Completeness [%] | 98.6 | 97.9 |
Redundancy | 6.5 | 6.9 |
CC(1/2) | 0.996 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% glycerol 22% PEG 1500 and additive: 0.02M HEPES sodium pH 6.8 and benzidine nicotinamide, pyromellitic acid and sulfaguanidine, each at 0.25% w/v |