5MCP
Structure of IMP dehydrogenase from Ashbya gossypii bound to ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 127.891, 152.090, 152.255 |
Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
Resolution | 152.042 - 2.400 |
R-factor | 0.2505 |
Rwork | 0.249 |
R-free | 0.27120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z0g |
RMSD bond length | 0.003 |
RMSD bond angle | 0.588 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 152.042 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.206 | 1.487 |
Number of reflections | 226714 | |
<I/σ(I)> | 5.8 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 6.3 |
CC(1/2) | 0.994 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.05 M Imidazole + 0.05 M Mes; pH 6.5 20 % Glycerol 20 % PEG-4000 0.02 M D-glucose 0.02 M D-Mannose 0.02 M D_Galactose 0.02 M L-Fucose 0.02 M C-Xylose 0.02 M N-Acetyl-D-Glucosamine |