5M80
Translation initiation factor 4E in complex with (RP)-iPr-m7GppSpG mRNA 5' cap analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 |
Unit cell lengths | 38.237, 38.271, 146.818 |
Unit cell angles | 87.53, 95.37, 102.94 |
Refinement procedure
Resolution | 37.290 - 2.120 |
R-factor | 0.2066 |
Rwork | 0.204 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.068 | 0.417 |
Number of reflections | 43091 | |
<I/σ(I)> | 10.47 | 2 |
Completeness [%] | 93.9 | 89.5 |
Redundancy | 1.979 | 1.829 |
CC(1/2) | 0.996 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 19% PEG 10000, 0.2M CH3COONa, 0.1M TRIS pH 8.0 |