5M5P

S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-16
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	P 21 21 2
Unit cell lengths	185.970, 196.870, 191.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	95.500 - 4.200
R-factor	0.3085
Rwork	0.307
R-free	0.33490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bgd
RMSD bond length	0.003
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	95.500
High resolution limit [Å]	4.200
Number of reflections	51802
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	10.8
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2