5M5P

S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Pre-mRNA-splicing helicase BRR2	polymer	1897	215817.0	2	UniProt (P32639) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF23445) Pfam (PF02889)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Protein Snu246
2	B, D (B, D)	Pre-mRNA-splicing factor 8	polymer	270	30490.3	2	UniProt (P33334) Pfam (PF08084)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Sequence modifications

A, C: 271 - 2163 (UniProt: P32639)

PDB	External Database	Details
Gly 267	-	expression tag
Ala 268	-	expression tag
Glu 269	-	expression tag
Phe 270	-	expression tag

B, D: 2147 - 2413 (UniProt: P33334)

PDB	External Database	Details
Gly 2144	-	expression tag
Ala 2145	-	expression tag
Met 2146	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	492614.7
All*	Total formula weight	492614.7

*Water molecules are not included.