5M3H

Bat influenza A/H17N10 polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97239
Spacegroup name	C 1 2 1
Unit cell lengths	269.490, 147.520, 88.480
Unit cell angles	90.00, 97.22, 90.00

Refinement procedure

Resolution	49.528 - 2.500
R-factor	0.2118
Rwork	0.210
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsb
RMSD bond length	0.002
RMSD bond angle	0.529
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.118	1.250
Number of reflections	117997
<I/σ(I)>	9.45	1.21
Completeness [%]	99.6	99.6
Redundancy	5.84	5.9
CC(1/2)	0.996	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	278	Bat influenza polymerase at 10 mgs per ml in 50 mM HEPES NaOH, 500 mM NaCl, 5% (v/v) glycerol, 2 mM TCEP at pH 7.5 with 1:1 ratio of vRNA promoter and CTD peptide mixed with 0.7-1.5 M sodium/potassium phosphate at pH 5.0