5M3H
Bat influenza A/H17N10 polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97239 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 269.490, 147.520, 88.480 |
Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
Resolution | 49.528 - 2.500 |
R-factor | 0.2118 |
Rwork | 0.210 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wsb |
RMSD bond length | 0.002 |
RMSD bond angle | 0.529 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.118 | 1.250 |
Number of reflections | 117997 | |
<I/σ(I)> | 9.45 | 1.21 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 5.84 | 5.9 |
CC(1/2) | 0.996 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 278 | Bat influenza polymerase at 10 mgs per ml in 50 mM HEPES NaOH, 500 mM NaCl, 5% (v/v) glycerol, 2 mM TCEP at pH 7.5 with 1:1 ratio of vRNA promoter and CTD peptide mixed with 0.7-1.5 M sodium/potassium phosphate at pH 5.0 |