5M1N
Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.804, 53.936, 70.079 |
Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
Resolution | 43.780 - 1.200 |
R-factor | 0.1697 |
Rwork | 0.169 |
R-free | 0.18220 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.780 | 43.780 | 1.220 |
High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
Rmerge | 0.042 | 0.028 | 0.337 |
Number of reflections | 96799 | ||
<I/σ(I)> | 17.7 | ||
Completeness [%] | 95.2 | 97.8 | 91.8 |
Redundancy | 4.3 | 4.1 | 4.4 |
CC(1/2) | 0.999 | 0.998 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Tartrate, 0.1 M Bis-tris pH 5.5-6.5, 20% PEG 3350 |