5LVP
Human PDK1 Kinase Domain in Complex with an HM-Peptide Bound to the PIF-Pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.830, 168.510, 94.880 |
| Unit cell angles | 90.00, 93.06, 90.00 |
Refinement procedure
| Resolution | 94.745 - 2.500 |
| R-factor | 0.1764 |
| Rwork | 0.174 |
| R-free | 0.22570 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 51591 |
| <I/σ(I)> | 12.5 |
| Completeness [%] | 99.7 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 1.65 M Ammonium Sulfate, 1 M Na Citrate pH 5.7, 0.01 M DTT |
