5LV5
Crystal structure of mouse PRMT6 in complex with inhibitor LH1458
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9801 |
Spacegroup name | I 41 |
Unit cell lengths | 77.703, 77.703, 117.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.160 - 1.802 |
R-factor | 0.1561 |
Rwork | 0.154 |
R-free | 0.19520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c07 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.160 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.105 | 1.261 |
Number of reflections | 32113 | |
<I/σ(I)> | 10.7 | 1.2 |
Completeness [%] | 99.8 | 97.3 |
Redundancy | 5.2 | 4.7 |
CC(1/2) | 0.998 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM PEG 6000 10 % MgCl2 200 mM |