5LV3
Crystal structure of mouse CARM1 in complex with ligand LH1561Br
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-11 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.092, 98.524, 208.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.992 - 1.800 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.156 | 1.867 |
| Number of reflections | 143500 | |
| <I/σ(I)> | 8.7 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 7.9 |
| CC(1/2) | 0.997 | 0.302 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | Tris-HCl pH 8.0 100 mM PEG 2000 MME 15% NaCl 100 mM |
