5LV2
Crystal structure of mouse CARM1 in complex with inhibitor LH1246
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.684, 74.832, 206.639 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.290 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ih3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.912 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1980) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.158 | 1.162 |
| Number of reflections | 134100 | |
| <I/σ(I)> | 5.5 | 1 |
| Completeness [%] | 99.1 | 90.9 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.985 | 0.412 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl pH 8.0 100 mM PEG 2000 MME 17 % NaCl 100 mM |
