5LTS

Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease Mutant D118A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	C 2 2 21
Unit cell lengths	144.825, 159.221, 52.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.610 - 2.510
R-factor	0.208
Rwork	0.207
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jsb
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.570	2.600
High resolution limit [Å]	2.510	2.510
Rmerge	0.060
Number of reflections	31183
<I/σ(I)>	14.17	2.09
Completeness [%]	99.0	100
Redundancy	3.5	3.4
CC(1/2)	0.997	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0,1M pH 6, Isopropanol 3,5%, MnCl2 1mM