5LTS
Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease Mutant D118A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.825, 159.221, 52.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.610 - 2.510 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.570 | 2.600 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.060 | |
| Number of reflections | 31183 | |
| <I/σ(I)> | 14.17 | 2.09 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.997 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0,1M pH 6, Isopropanol 3,5%, MnCl2 1mM |
