5LTS

Crystal structure of Lymphocytic choriomeningitis mammarenavirus endonuclease Mutant D118A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RNA-directed RNA polymerase L	polymer	204	23678.2	2	UniProt (A0A059U382) Pfam (PF17296) UniProt (by SIFTS) (P14240) In PDB	Lymphocytic choriomeningitis mammarenavirus	Protein L
2	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	B	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5		water	water		18.0	113	Chemie (HOH)

Sequence modifications

A, B: 1 - 196 (UniProt: A0A059U382)

PDB	External Database	Details
Met -7	-	initiating methionine
Lys -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 118	Asp 119	engineered mutation
Asp 173	Asn 174	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	47356.3
Non-Polymers*	Number of molecules	3
	Total formula weight	150.5
All*	Total formula weight	47506.9

*Water molecules are not included.