5LSJ
CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C delta-HEAD2 COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.160, 156.400, 76.000 |
Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
Resolution | 19.993 - 3.250 |
R-factor | 0.2488 |
Rwork | 0.246 |
R-free | 0.29710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lsk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.815 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.500 | 3.080 | |
High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
Rmerge | 0.351 | 0.085 | 1.026 |
Number of reflections | 31073 | ||
<I/σ(I)> | 9.47 | 32.6 | 3.89 |
Completeness [%] | 99.9 | 96 | 100 |
Redundancy | 23.7 | 24.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 20% PEG3350, 0.2M potassium sodium tartrate |