5LRW
Structure of Cezanne/OTUD7B OTU domain bound to ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | H 3 |
Unit cell lengths | 157.560, 157.560, 75.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.484 - 2.000 |
R-factor | 0.1781 |
Rwork | 0.176 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.623 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.130 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.125 | 0.854 |
Number of reflections | 47250 | |
<I/σ(I)> | 8.6 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate (pH 4.8), 6% (w/v) PEG 6K |