5LOW
Structure of the Ca2+-bound Rabphilin 3A C2B domain SNAP25 complex (P21 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.080, 239.496, 71.487 |
| Unit cell angles | 90.00, 114.92, 90.00 |
Refinement procedure
| Resolution | 45.030 - 2.800 |
| R-factor | 0.23944 |
| Rwork | 0.238 |
| R-free | 0.26857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lob |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.173 | |
| Number of reflections | 43944 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 98.3 | 96.2 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG3350 0.35 M Ammonium sulfate 0.1 M Tris (pH 8.5) al's oil |
