5LIQ
The structure of C160S,C508S,C578S mutant of Nt.BspD6I nicking endonuclease at 0.185 nm resolution .
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.204, 92.485, 113.808 |
| Unit cell angles | 90.00, 105.67, 90.00 |
Refinement procedure
| Resolution | 19.580 - 1.850 |
| R-factor | 0.21116 |
| Rwork | 0.209 |
| R-free | 0.25143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.943 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.600 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.033 | 0.660 |
| Number of reflections | 129563 | |
| <I/σ(I)> | 18.7 | 2 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 16% PEG 8000 40MM POTASSIUM PHOSPHATE 20% GLYCEROL |
