5LIQ

The structure of C160S,C508S,C578S mutant of Nt.BspD6I nicking endonuclease at 0.185 nm resolution .

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Nicking endonuclease N.BspD6I	polymer	610	71653.9	2	UniProt (Q8GCA3) Pfam (PF09491) In PDB	Bacillus sp.
2	A, B	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
3	A, B	PHOSPHATE ION	non-polymer		95.0	7	Chemie (PO4)
4		water	water		18.0	295	Chemie (HOH)

Sequence modifications

A, B: 1 - 604 (UniProt: Q8GCA3)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 160	Cys 160	engineered mutation
Ser 508	Cys 508	engineered mutation
Ser 578	Cys 578	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143307.8
Non-Polymers*	Number of molecules	15
	Total formula weight	1401.5
All*	Total formula weight	144709.3

*Water molecules are not included.