5LHQ
The EGR-cmk active site inhibited catalytic domain of murine urokinase-type plasminogen activator in complex with the allosteric inhibitory nanobody Nb7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 94.250, 94.250, 122.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.811 - 2.600 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5LHR and 4JVP |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.820 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.087 | 0.966 |
| Number of reflections | 19919 | |
| <I/σ(I)> | 21.91 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 288 | 100 mM HEPES, 1.6 M Li2SO4 |
