5LHC

The structure of D456A mutant of Nt.BspD6I nicking endonuclease at 0.24 nm resolution .

Replaces:  4WL5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.96863
Spacegroup name	P 1 21 1
Unit cell lengths	76.350, 92.666, 113.969
Unit cell angles	90.00, 105.75, 90.00

Refinement procedure

Resolution	19.000 - 2.400
R-factor	0.19872
Rwork	0.195
R-free	0.26539
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ewf
RMSD bond length	0.015
RMSD bond angle	1.780
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.700	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.610
Number of reflections	59826
<I/σ(I)>	9.8
Completeness [%]	99.5	99.4
Redundancy	3.6	3.5
CC(1/2)		0.024

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	16% PEG 8000 40MM POTASSIUM PHOSPHATE 20% GLYCEROL